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The collagen fraction usually yields more reliable dates than the apatite fraction (see Dates on bones).

In addition to various pre-treatments, the sample must be burned and converted to a form suitable for the counter.

This discovery meant that there are three naturally occurring isotopes of carbon: Whereas carbon-12 and carbon-13 are stable isotopes, carbon-14 is unstable or radioactive.

Carbon-14 is produced in the upper atmosphere when cosmic rays bombard nitrogen atoms.

Most samples require chemical pre-treatment to ensure their purity or to recover particular components of the material.

The objective of pre-treatment is to ensure that the carbon being analyzed is native to the sample submitted for dating.

Since there are practical limits to the age range of the method, most samples must be younger than 50,000 years and older than 100 years.These so-called "solid-carbon" dates were soon found to yield ages somewhat younger than expected, and there were many other technical problems associated with sample preparation and the operation of the counters.Gas proportional counters soon replaced the solid-carbon method in all laboratories, with the samples being converted to gases such as carbon dioxide, carbon disulfide, methane, or acetylene.Libby calculated the half-life of c14 as 5568 ± 30 years.This means that half of the c14 has decayed by the time an organism has been dead for 5568 years, and half of the remainder has decayed by 11,136 years after death, etc.

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