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Research has been ongoing since the 1960s to determine what the proportion of in the atmosphere has been over the past fifty thousand years.

The resulting data, in the form of a calibration curve, is now used to convert a given measurement of radiocarbon in a sample into an estimate of the sample's calendar age.

Neither the pre-existing Egyptian chronology nor the new radiocarbon dating method could be assumed to be accurate, but a third possibility was that the In the 1960s, Hans Suess was able to use the tree-ring sequence to show that the dates derived from radiocarbon were consistent with the dates assigned by Egyptologists.

This was possible because although annual plants, such as corn, have a The New Zealand curve is representative of the Southern Hemisphere; the Austrian curve is representative of the Northern Hemisphere.

Libby and James Arnold proceeded to test the radiocarbon dating theory by analyzing samples with known ages.

This was revised in the early 1960s to 5,730 years, which meant that many calculated dates in papers published prior to this were incorrect (the error in the half-life is about 3%).In 1939, Martin Kamen and Samuel Ruben of the Radiation Laboratory at Berkeley began experiments to determine if any of the elements common in organic matter had isotopes with half-lives long enough to be of value in biomedical research.They synthesized Libby and several collaborators proceeded to experiment with methane collected from sewage works in Baltimore, and after isotopically enriching their samples they were able to demonstrate that they contained radioactive .Other corrections must be made to account for the proportion of throughout the biosphere (reservoir effects).Additional complications come from the burning of fossil fuels such as coal and oil, and from the above-ground nuclear tests done in the 1950s and 1960s.

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